Plastic origami (plastic folder source) worked well to make specimen holder. 2 widths possible by either folding down on bottom to furthest stop or by placing bottom together in slit cut. Slide holder is filled with water and specimen is hand advanced with the slightest of nudges over the length of the specimen holder. Cuts are made on the other side of the glass slide, which holds the specimen holder in place and also acts to create a smooth cutting surface for better quality cut (using standard razor blade). The glass slide has a semi-ovular cut from the bottom using a steel wheel glass cutter (see video). This cut allows the specimen to advance beyond the glass so that the specimen can be cut. Cross sectional cuts of stem are picked up with needle nose forceps and placed on a slide. Extra water was absorbed with Kim wipes separating slides. Clear tape is used as cover slip to secure specimen to slide and then specimen are examined using Carson handheld microscope. ...
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